Active Ingredient: Ciprofloxacin
Tissue imaging and analysis were performed by the NanoZoomer 2.
Hamamatsu, Japan. The same acquisition setting, including scaling, applies to all images in the same figure. The mucosal damage of ileum for each slide was graded on a six-tiered index defined by Chiu et al.
The bone marrow cellularity was assessed by a board certified veterinary pathologist in a blinded manner through the averaging of total erythroid and myeloid cells over 10 high-powered fields, subsequent to a low power qualitative assessment.
The megakaryocytes were also counted and averaged over 10 high-powered fields. Since regenerative foci can be unbalanced within a section, they were included in the measurement over 10 fields based on the low power overview of the whole section.
In some studies, paraffin sections were also used for the staining as noted. Intensity of signals were also measured and shown as noted.
Cytokine and Chemokine Measurements Whole blood 0. Briefly, serum from each animal was diluted fourfold and examined in duplicate.
Caspase in the lysates cleaved the substrate to form pNA as indicated by an increase in absorbance at 405 nm, which was measured using a SpectraMax 250 spectrophotometric plate reader and SOFTmax Pro 3.
Detection of Bacterial Translocation The detailed methods used for detecting bacteria in blood and tissues to assess bacterial translocation were previously described.Published online Mar 8. PLoS One.
Briefly, mice were dissected aseptically to isolate facultative bacteria from selected tissues. Samples of liver, spleen and ileum were removed and homogenized by crushing with a sterile cotton or polyester swab in a sterile Petri dish and inoculated immediately onto SBA, CNA, and XLD media.
Acknowledgments We thank Ms. Joan T. Smith, HM 1 Neil G. Agravante, USN, Mr.
True M. Burns, Dr. Min Zhai, and Dr. During recolonization, the bacterial counts remained lower time, the selection of antibiotic resistance in significantly, and almost reached pre-treatment levels in genes of resistance, during the prolonged exposure swine model.