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To date, the only commercially feasible technology to produce azadirachtin is by natural seed extraction, but there are some serious drawbacks to this ap- proach.

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Seeds are known to loose consider- Chapter 12 In Vitro Azadirachtin Production 243 able viability and azadirachtin content during storage. Heterogeneity in azadirachtin content in seeds has also been reported by Sidhu and Behl. Moreover, the current supply of azadirachtin is unable to meet the increasing demands.

Hence, there is a need for the development of a commercially viable alternative for its enhanced and continuous production. To make secondary metabolite production economically feasible, proper selec- tion and establishment of a high-yielding elite cell or tissue culture system is a necessary prerequisite.

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All of these studies exhibited variations in the callus induction response depending upon the age and size of explants and cultural conditions employed among the diverse neem genotype. Azadirachtin could not be detected in certain in vitro propagated shoot systems.

The formation of transformed callus using Agrobacterium tumefaciens has also been reported.

However, azadirachtin production from the transformed callus has not been reported to date. From an engi- neering perspective, cell suspension cultures have more immediate potential for industrial application.

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